Author: Aravinth Kumar Jayabalan; Diane E. Griffin; Anthony K. L. Leung
Title: Alphavirus nsP3 ADP-ribosylhydrolase Activity Disrupts Stress Granule Formation Document date: 2019_6_20
ID: n8sjpcbs_23
Snippet: CHIKV WT and nsP3 mutants were examined 5.5 h after infection when nsP3 appears as foci in the absence of arsenite (Fig. S4a) . To examine the ability of WT, G32S and Y114A nsP3s to suppress SG formation, infected cells were treated with arsenite for 0.5 h (a time point when ~95% of uninfected cells have developed SGs; data not shown). Consistent with experiments using nsP3 protein expression constructs (Fig. 2a, b) , WT CHIKV infection suppresse.....
Document: CHIKV WT and nsP3 mutants were examined 5.5 h after infection when nsP3 appears as foci in the absence of arsenite (Fig. S4a) . To examine the ability of WT, G32S and Y114A nsP3s to suppress SG formation, infected cells were treated with arsenite for 0.5 h (a time point when ~95% of uninfected cells have developed SGs; data not shown). Consistent with experiments using nsP3 protein expression constructs (Fig. 2a, b) , WT CHIKV infection suppressed SG formation, where only 27% of nsP3-positive cells had canonical (G3BP1/eIF3b double-positive) SGs (Fig. 3a, b) . All rights reserved. No reuse allowed without permission.
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