Ultra-Low-Cost Integrated Silicon-based Transducer for On-Site, Genetic Detection of Pathogens

Estefania Nunez-Bajo; Michael Kasimatis; Yasin Cotur; Tarek Asfour; Alex Collins; Ugur Tanriverdi; Max Grell; Matti Kaisti; Guglielmo Senesi; Karen Stevenson; Firat Guder

Using the TriSilix chip, we have performed real-time and quantitative RPA (isothermal, qRPA) and PCR (cyclic, qPCR) amplification of DNA using its temperature transduction capabilities and Figure 5B ). Once again, we performed SWV as the analytical method and measured anodic and cathodic peak current intensities originating from MB as the electroanalytical signal. Without DNA amplification, we were able to detect genomic DNA of MAP K10 (4,829 kbp) down to an LoD of 0.8 pg from cathodic peak current intensities in comparison to a LoD of 1.2 pg provided by the anodic peak current intensities; the cathodic peak current intensity was, therefore, used as the electroanalytical signal in the following PCR experiments. We performed qPCR using the TriSilix chip to amplify and quantify small amounts (0 -500 fg) of DNA of MAP K10 in real-time ( Figure 5C ). The forward primer (5´-GCC GCG CTG CTG GAG TTG A-3´) and reverse author/funder. All rights reserved. No reuse allowed without permission.