Ultra-Low-Cost Integrated Silicon-based Transducer for On-Site, Genetic Detection of Pathogens

Estefania Nunez-Bajo; Michael Kasimatis; Yasin Cotur; Tarek Asfour; Alex Collins; Ugur Tanriverdi; Max Grell; Matti Kaisti; Guglielmo Senesi; Karen Stevenson; Firat Guder

The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.23.002931 doi: bioRxiv preprint 13 primer (5'-CGC GGC ACG GCT CTT GTT -3´), were used to amplify a 563 nucleotide segment of IS900 (204-766 of GenBank Accesion Number AE016958.1; National Center for Biotechnology Information, USA), which has 17 repeats in the genome of MAP K10. Using the qPCR approach shown in Figure S7 , we were able to measure as low as 20 fg of genomic DNA of MAP K10 which is equivalent to detection of a single bacterium in the sample. We also characterized the same samples using a commercial laboratory qPCR (IDvet, UK) using a test manufactured by ID Gene TM as a goldstandard ( Figure S8 ). The results produced by TriSilix were similar or better in comparison to those produced by the commercially available, sophisticated laboratory instrument; for 20 fg of MAP K10 DNA, the resulting Ct (cycle threshold) value was 30 using TriSilix, 5 cycles shorter compared to the value obtained using the commercial qPCR (Ct=35).