Author: Dorothea Bestle; Miriam Ruth Heindl; Hannah Limburg; Thuy Van Lam van; Oliver Pilgram; Hong Moulton; David A. Stein; Kornelia Hardes; Markus Eickmann; Olga Dolnik; Cornelius Rohde; Stephan Becker; Hans-Dieter Klenk; Wolfgang Garten; Torsten Steinmetzer; Eva Böttcher-Friebertshäuser
Title: TMPRSS2 and furin are both essential for proteolytic activation and spread of SARS-CoV-2 in human airway epithelial cells and provide promising drug targets Document date: 2020_4_15
ID: anedg12x_30
Snippet: Expression plasmid pCAGGS-TMPRSS2 encoding the cDNA of human TMPRSS2 has been 576 described previously (Böttcher et al., 2006) . . The S1/S2 and S2′ cleavage sites and subunits S1, S2 and S2' are indicated by black and coloured arrows, respectively. For immunochemical detection recombinant S is expressed with a C-terminally fused Myc-6xHis-tag peptide in our study. B) Alignment of the amino acid sequences at the S1/S2 and S2' cleavage site of .....
Document: Expression plasmid pCAGGS-TMPRSS2 encoding the cDNA of human TMPRSS2 has been 576 described previously (Böttcher et al., 2006) . . The S1/S2 and S2′ cleavage sites and subunits S1, S2 and S2' are indicated by black and coloured arrows, respectively. For immunochemical detection recombinant S is expressed with a C-terminally fused Myc-6xHis-tag peptide in our study. B) Alignment of the amino acid sequences at the S1/S2 and S2' cleavage site of the S proteins of different human coronaviruses (HCoV) and avian infectious bronchitis virus (IBV) strain Beaudette.
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