Efficacy of marine cyanobacterium Oxynema thaianum ALU PBC5 against Multi Drug Resistant Gram Negative Pathogens.

Nagarajan, Padmini; Antony Alex Kennedy, Ajilda; Pandiyan, Prabhakaran; Natesan, Sivakumar; Gopal, Selvakumar

AIM Emergence of extended antibiotic resistance among several human bacterial pathogens often leads to the failure of existing antibiotics to treat bacterial infections worldwide. Hence, the present study is aimed to explore anti-bacterial activity of marine cyanobacterium against MDR pathogens. METHODS AND RESULTS The cyanobacterial samples were collected and isolated from Thondi Palk Strait region. The isolate was subjected to polarity based solvent extraction and checked for their anti-bacterial activity against test bacterial pathogens. The active principles from chloroform extract of Oxynema thaianum (CEOT) were partially purified through TLC. The active principle with highest activity was further characterized by FTIR, HPLC and GC-MS analysis. Among the eight extracts tested, CEOT showed effective zone of clearance against ESBL producing E. coli and K. pneumoniae in disc diffusion method. In TLC, all the purified five fractions were eluted and tested for their anti-bacterial activity against test pathogens. The third fraction showing maximum activity was subjected to HPLC analysis for checking its purity. In GC-MS analysis, 9-Octadecenoic acid, methyl ester and Hexadecanoic acid were identified as the major chemical compounds. CONCLUSION Hence, the present study was concluded that O. thaianum ALU PBC5 is a promising agent to treat ESBL producing MDR bacterial pathogens.