Selected article for: "cell line and extract ml"
Author: Sa-ngiamsuntorn, Khanit; Suksatu, Ampa; Pewkliang, Yongyut; Thongsri, Piyanoot; Kanjanasirirat, Phongthon; Manopwisedjaroen, Suwimon; Charoensutthivarakul, Sitthivut; Wongtrakoongate, Patompon; Pitiporn, Supaporn; Chaopreecha, Jarinya; Kongsomros, Supasek; Jearawuttanakul, Kedchin; Wannalo, Warawuth; Khemawoot, Phisit; Chutipongtanate, Somchai; Borwornpinyo, Suparerk; Thitithanyanont, Arunee; Hongeng, Suradej
Title: Anti-SARS-CoV-2 Activity of Andrographis paniculata Extract and Its Major Component Andrographolide in Human Lung Epithelial Cells and Cytotoxicity Evaluation in Major Organ Cell Representatives
  • Cord-id: dfinv0po
  • Document date: 2021_4_12
    • ID: dfinv0po
    Snippet: [Image: see text] The coronaviruses disease 2019 (COVID-19) caused by a novel coronavirus (SARS-CoV-2) has become a major health problem, affecting more than 50 million people with over one million deaths globally. Effective antivirals are still lacking. Here, we optimized a high-content imaging platform and the plaque assay for viral output study using the legitimate model of human lung epithelial cells, Calu-3, to determine the anti-SARS-CoV-2 activity of Andrographis paniculata extract and it
    Document: [Image: see text] The coronaviruses disease 2019 (COVID-19) caused by a novel coronavirus (SARS-CoV-2) has become a major health problem, affecting more than 50 million people with over one million deaths globally. Effective antivirals are still lacking. Here, we optimized a high-content imaging platform and the plaque assay for viral output study using the legitimate model of human lung epithelial cells, Calu-3, to determine the anti-SARS-CoV-2 activity of Andrographis paniculata extract and its major component, andrographolide. SARS-CoV-2 at 25TCID(50) was able to reach the maximal infectivity of 95% in Calu-3 cells. Postinfection treatment of A. paniculata and andrographolide in SARS-CoV-2-infected Calu-3 cells significantly inhibited the production of infectious virions with an IC(50) of 0.036 μg/mL and 0.034 μM, respectively, as determined by the plaque assay. The cytotoxicity profile developed over the cell line representatives of major organs, including liver (HepG2 and imHC), kidney (HK-2), intestine (Caco-2), lung (Calu-3), and brain (SH-SY5Y), showed a CC(50) of >100 μg/mL for A. paniculata extract and 13.2–81.5 μM for andrographolide, respectively, corresponding to a selectivity index of over 380. In conclusion, this study provided experimental evidence in favor of A. paniculata and andrographolide for further development as a monotherapy or in combination with other effective drugs against SARS-CoV-2 infection.

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