Author: Chen, Chuan; Sun, Zehua; Liu, Xianglei; Li, Wei; Dimitrov, Dimiter S.
Title: Protocol for constructing large size human antibody heavy chain variable domain (VH) library and selection of SARS-CoV-2 neutralizing antibody domains Cord-id: 7wdtr5hx Document date: 2021_5_29
ID: 7wdtr5hx
Snippet: This protocol is a comprehensive guide to phage display based selection of virus neutralizing VH antibody domains. It details three optimized parts including 1) Construction of large size (theoretically > 1011) naïve human antibody heavy chain domain library, 2) SARS-COV-2 antigen expression and stable cell line construction, and 3) Library panning for selection of SARS-CoV-2 specific antibody domains. Using this protocol, we identified a high-affinity neutralizing human VH antibody domain, VH
Document: This protocol is a comprehensive guide to phage display based selection of virus neutralizing VH antibody domains. It details three optimized parts including 1) Construction of large size (theoretically > 1011) naïve human antibody heavy chain domain library, 2) SARS-COV-2 antigen expression and stable cell line construction, and 3) Library panning for selection of SARS-CoV-2 specific antibody domains. Using this protocol, we identified a high-affinity neutralizing human VH antibody domain, VH ab8, which exhibits high prophylactic and therapeutic efficacy.
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