Author: Natali Ozber; Paolo Margaria; Charles T. Anderson; Massimo Turina; Cristina Rosa
Title: The role of post-Golgi transport pathways and sorting motifs in the plasmodesmal targeting of the movement protein (MP) of Ourmia melon virus (OuMV) Document date: 2019_8_11
ID: bvcahbbi_43
Snippet: The OuMV infectious clone, consisting of the three plasmids pGC-RNA1 (RdRP), pGC-RNA2 (MP) and pGC-RNA3 (CP), and GFP:MP plasmid has been previously described (Crivelli et al., 2011; Margaria et al., 2016) . Mutations were inserted into MP and GFP:MP plasmid with the QuickChange Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA, USA) using primers in Table S1 ......
Document: The OuMV infectious clone, consisting of the three plasmids pGC-RNA1 (RdRP), pGC-RNA2 (MP) and pGC-RNA3 (CP), and GFP:MP plasmid has been previously described (Crivelli et al., 2011; Margaria et al., 2016) . Mutations were inserted into MP and GFP:MP plasmid with the QuickChange Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA, USA) using primers in Table S1 .
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