The Essential Facts of Wuhan Novel Coronavirus Outbreak in China and Epitope-based Vaccine Designing against COVID-19

Bishajit Sarkar; Md. Asad Ullah; Fatema Tuz Johora; Masuma Afrin Taniya; Yusha Araf

From the secondary structure analysis, it was determined that, the CV-1 had the highest percentage of the amino acids (67.1%) in the coil formation as well as the highest percentage of amino acids (8%) in the beta-strand formation. However, CV-3 had the highest percentage of 37.8% of amino acids in the alpha-helix formation (Figure 05 and Table 13 ). CV-1 and CV-2 vaccines had 02 domains, whereas, CV-3 had only one domain. CV-2 had the lowest p-value of 6.35e-05. The pvalue represents the relative quality of a protein model. The smaller p-value refers to higher quality of the protein model and vice-verse [176] . For this reason, CV-2 showed the best performance in the 3D structure generation experiment. Moreover, three different templates were used for generating3D structures of the three different vaccines. The RaptorX server used the templates for generating the 3D structures of the query vaccine constructs [177] . The results of the 3D structure analysis are listed in Table 14 and illustrated in Figure 06 .