Author: Anastassia K. Pogoutse; Trevor F. Moraes
Title: Transferrin binding protein B and Transferrin binding protein A 2 expand the transferrin recognition range of Histophilus somni Document date: 2019_8_9
ID: 2chmny8w_5
Snippet: Control TbpAs from the ruminant pathogen Mannheimia haemolytica and from the porcine 169 pathogen Actinobacillus pleuropneumoniae were used to validate the assay. The TbpAs from 170 these pathogens bound specifically to their cognate transferrins in our assay. We used a similar 171 approach to evaluate whether HsTbpB binding was specific for bovine transferrin. A 172 displacement ELISA using soluble GST-HsTbpB showed that unlike HsTbpA, HsTbpB bi.....
Document: Control TbpAs from the ruminant pathogen Mannheimia haemolytica and from the porcine 169 pathogen Actinobacillus pleuropneumoniae were used to validate the assay. The TbpAs from 170 these pathogens bound specifically to their cognate transferrins in our assay. We used a similar 171 approach to evaluate whether HsTbpB binding was specific for bovine transferrin. A 172 displacement ELISA using soluble GST-HsTbpB showed that unlike HsTbpA, HsTbpB binds 173 bovine, ovine, and caprine transferrins, but has higher affinity for bovine transferrin ( Figure 2C ). 174
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