Author: D. Molodenskiy; H. Mertens; D. Svergun
Title: An automated data processing and analysis pipeline for transmembrane proteins in detergent solutions Document date: 2019_7_24
ID: lhriiv4u_9
Snippet: The automated membrane protein pipeline (AMPP) described herein, is implemented at the P12 beamline of the EMBL at Petra-III storage ring (synchrotron DESY, Hamburg, Germany) as an extension of a standard SEC-SAXS pipeline SASFLOW. It is integrated into the beamline control software BECQUEREL (29) operating the beamline. If the MP-related fields in the ISPyB database are lacking, BECQUEREL runs a standard pipeline mode for soluble proteins, using.....
Document: The automated membrane protein pipeline (AMPP) described herein, is implemented at the P12 beamline of the EMBL at Petra-III storage ring (synchrotron DESY, Hamburg, Germany) as an extension of a standard SEC-SAXS pipeline SASFLOW. It is integrated into the beamline control software BECQUEREL (29) operating the beamline. If the MP-related fields in the ISPyB database are lacking, BECQUEREL runs a standard pipeline mode for soluble proteins, using the program DAMMIF (30) for a single-phase ab initio shape determination. The MPrelated extension starts by default if the connection with ISPyB is established by the control software, and the membrane-specific fields in the ISPyB database are filled. The system has been optimized through extensive testing on different MPs in detergent solutions, and several examples of its applications are presented below.
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