The role of post-Golgi transport pathways and sorting motifs in the plasmodesmal targeting of the movement protein (MP) of Ourmia melon virus (OuMV)

Natali Ozber; Paolo Margaria; Charles T. Anderson; Massimo Turina; Cristina Rosa

. CC-BY-NC-ND 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/724716 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/724716 doi: bioRxiv preprint (MP) with indicated mutations, or sterile water (mock) at 14 dpi. White asterisks denote leaves shown in close-up images. Black arrowheads indicate mosaic symptoms on N. benthamiana infected with OuMV carrying MP_D/G. (D) Western blot analysis of agroinfiltrated leaves at 3 dpi (bottom panel) and upper uninoculated leaves at 14 dpi (top panel). CBB: Coomassie Brilliant Blue, loading control. (E) Replication assay of OuMV carrying Y and LL motif MP mutants at 48 hpi. Two leaf spots from 3 leaves were pooled for each sample at 24 hpi and 48 hpi. The qPCR was performed in duplicates. The 2 -ΔΔCT method was used to asses fold changes in replication of wild-type and mutants. The data was normalized first using 18S ribosomal RNA, and then a wild-type control at 24 hpi showing the lowest expression. Bar graph represents normalized fold changes (mean±SE, n=4). • , P <0.1; *, P <0.05; **P <0.01; ***, P < 0.001 by ANOVA.