Author: Junan Zhu; Kristina Rivera; Dror Baron
Title: Noisy Pooled PCR for Virus Testing Document date: 2020_4_8
ID: 8kccpd4x_1
Snippet: Motivation. Reverse transcription polymerase chain reaction (RT-PCR) is a prevalent diagnostic tool for infectious diseases, like coronavirus disease 2019 (COVID-19). RT-PCR is a labor intensive technical procedure that requires numerous trained laboratory personnel to analyze one patient sample [1] . Briefly, ribonucleic acid (RNA) is isolated from a patient's respiratory tract and purified for reverse transcription, a process where the RNA temp.....
Document: Motivation. Reverse transcription polymerase chain reaction (RT-PCR) is a prevalent diagnostic tool for infectious diseases, like coronavirus disease 2019 (COVID-19). RT-PCR is a labor intensive technical procedure that requires numerous trained laboratory personnel to analyze one patient sample [1] . Briefly, ribonucleic acid (RNA) is isolated from a patient's respiratory tract and purified for reverse transcription, a process where the RNA template is turned into complementary deoxyribonucleic acid (cDNA). cDNA, along with specific viral primers, is loaded into a machine, where cDNA is amplified and annealed to the target sequence. While extending through each PCR cycle, a reporter dye is cleaved or broken from a probe to amplify fluorescence intensity and reveal a positive sample.
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