Author: LaRinda A Holland; Emily A Kaelin; Rabia Maqsood; Bereket Estifanos; Lily I Wu; Arvind Varsani; Rolf U Halden; Brenda G Hogue; Matthew Scotch; Efrem S Lim
Title: An 81 base-pair deletion in SARS-CoV-2 ORF7a identified from sentinel surveillance in Arizona (Jan-Mar 2020) Document date: 2020_4_22
ID: mkcrl9pw_6
Snippet: Arizona. Individuals presenting with respiratory symptoms (ILI) were tested for influenza A and 31 B virus (Alere BinaxNOW). Subsequently, we tested influenza-negative nasopharyngeal (NP) 32 swabs for SARS-CoV-2. We extracted total nucleic acid using the bioMérieux eMAG automated 33 platform and performed real-time RT-PCR (qRT-PCR) assays specific for SARS-CoV-2 N and E 34 genes (2, 3). Out of 382 NP swabs collected from January 24, 2020 to Marc.....
Document: Arizona. Individuals presenting with respiratory symptoms (ILI) were tested for influenza A and 31 B virus (Alere BinaxNOW). Subsequently, we tested influenza-negative nasopharyngeal (NP) 32 swabs for SARS-CoV-2. We extracted total nucleic acid using the bioMérieux eMAG automated 33 platform and performed real-time RT-PCR (qRT-PCR) assays specific for SARS-CoV-2 N and E 34 genes (2, 3). Out of 382 NP swabs collected from January 24, 2020 to March 25, 2020, we 35 detected SARS-CoV-2 in 5 swabs in the week of March 16 to 19 (Figure 1 ). This corresponds 36 to prevalence of 1.31%. Given the estimated 1 -14-day incubation period for COVID-19, it is 37 possible that the spike in cases might be related to university spring-break holiday travel (March 38 8 -15) as previously seen in other outbreaks (4, 5). 39
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