Author: Niccolo Alfano; Anisha Dayaram; Jan Axtner; Kyriakos Tsangaras; Marie-Louise Kampmann; Azlan Mohamed; Seth Timothy Wong; M. Thomas P. Gilbert; Andreas Wilting; Alex Daivd Greenwood
Title: Non-invasive surveys of mammalian viruses using environmental DNA Document date: 2020_3_29
ID: nil1vv6h_44
Snippet: Host identification of leeches followed an eDNA/iDNA workflow recently published [31] . In summary, leech samples were digested and short fragments of the mitochondrial markers 12S, 16S and cytochrome B were amplified in four PCR replicates each resulting in 12 PCR replicates per sample. We used a twin-tagging 2-step PCR protocol and PCR products were sequenced using an Illumina MiSeq (for details please see Axtner et al. 2019 [31] ). After demul.....
Document: Host identification of leeches followed an eDNA/iDNA workflow recently published [31] . In summary, leech samples were digested and short fragments of the mitochondrial markers 12S, 16S and cytochrome B were amplified in four PCR replicates each resulting in 12 PCR replicates per sample. We used a twin-tagging 2-step PCR protocol and PCR products were sequenced using an Illumina MiSeq (for details please see Axtner et al. 2019 [31] ). After demultiplexing and read processing, each haplotype was taxonomically assigned to a curated reference database using PROTAX [46] . Taxonomic assignments followed the criteria of Axtner et al. 2019.
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