Pre-Clinical Assessment of Single-Use Negative Pressure Wound Therapy During In Vivo Porcine Wound Healing.

Brownhill, Varuni R; Huddleston, Elizabeth; Bell, Andrea; Hart, Jeffrey; Webster, Iain; Hardman, Matthew; Wilkinson, Holly Nicola

Objective: Traditional negative pressure wound therapy systems can be large and cumbersome, limiting patient mobility and adversely affecting quality of life. PICOâ„¢, a no canister single-use system offers a lightweight, portable alternative to traditional negative pressure wound therapy, with improved clinical performance. The aim of this study was to determine the potential mechanism(s) of action of single-use negative pressure wound therapy versus traditional negative pressure wound therapy. Approach: Single-use negative pressure wound therapy and traditional negative pressure wound therapy were applied to an in vivo porcine excisional wound model, following product use guidelines. Macroscopic, histological and biochemical analyses were performed at defined healing time-points to assess multiple aspects of the healing response. Results: Wounds treated with single-use negative pressure displayed greater wound closure and increased re-epithelialisation versus those treated with traditional negative pressure. The resulting granulation tissue was more advanced with fewer neutrophils, reduced inflammatory markers, more mature collagen and no wound filler-associated foreign body reactions. Of note, single-use negative pressure therapy failed to induce wound edge epithelial hyperproliferation, while traditional negative pressure therapy compromised peri-wound skin, which remained inflamed with high transepidermal water loss; features not observed following single-use treatment. Innovation: Single-use negative pressure was identified to improve multiple aspects of healing versus traditional negative pressure treatment. Conclusion: This study provides important new insight into the differing mode of action of single-use versus traditional negative pressure and may go some way to explaining the improved clinical outcomes observed with single use negative pressure therapy.