Author: Erik Procko
Title: The sequence of human ACE2 is suboptimal for binding the S spike protein of SARS coronavirus 2 Document date: 2020_3_17
ID: jalijjmg_7
Snippet: Cells expressing ACE2 variants with high or low binding to RBD were collected by 69 fluorescence-activated cell sorting (FACS), referred to as "nCoV-S-High" and "nCoV-S-Low" 70 sorted populations, respectively. During FACS, fluorescence signal for bound RBD-sfGFP 71 continuously declined, requiring the collection gates to be regularly updated to 'chase' the 72 relevant populations. This is consistent with RBD dissociating over hours during the 73.....
Document: Cells expressing ACE2 variants with high or low binding to RBD were collected by 69 fluorescence-activated cell sorting (FACS), referred to as "nCoV-S-High" and "nCoV-S-Low" 70 sorted populations, respectively. During FACS, fluorescence signal for bound RBD-sfGFP 71 continuously declined, requiring the collection gates to be regularly updated to 'chase' the 72 relevant populations. This is consistent with RBD dissociating over hours during the 73 experiment. Reported affinities of RBD for ACE2 range from 1 to 15 nM (8, 10). 74 . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.16.994236 doi: bioRxiv preprint
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