Author: Natarajan, Aravind; Han, Alvin; Zlitni, Soumaya; Brooks, Erin F.; Vance, Summer E.; Wolfe, Marlene; Singh, Upinder; Jagannathan, Prasanna; Pinsky, Benjamin A.; Boehm, Alexandria; Bhatt, Ami S.
Title: Standardized preservation, extraction and quantification techniques for detection of fecal SARS-CoV-2 RNA Cord-id: ev04qy0o Document date: 2021_10_1
ID: ev04qy0o
Snippet: Patients with COVID-19 shed SARS-CoV-2 RNA in stool, sometimes well after their respiratory infection has cleared. This may be significant for patient health, epidemiology, and diagnosis. However, methods to preserve stool, and to extract and quantify viral RNA are not standardized. We test the performance of three preservative approaches at yielding detectable SARS-CoV-2 RNA: the OMNIgene-GUT kit, Zymo DNA/RNA shield kit, and the most commonly applied, storage without preservative. We test thes
Document: Patients with COVID-19 shed SARS-CoV-2 RNA in stool, sometimes well after their respiratory infection has cleared. This may be significant for patient health, epidemiology, and diagnosis. However, methods to preserve stool, and to extract and quantify viral RNA are not standardized. We test the performance of three preservative approaches at yielding detectable SARS-CoV-2 RNA: the OMNIgene-GUT kit, Zymo DNA/RNA shield kit, and the most commonly applied, storage without preservative. We test these in combination with three extraction kits: QIAamp Viral RNA Mini Kit, Zymo Quick-RNA Viral Kit, and MagMAX Viral/Pathogen Kit. We also test the utility of ddPCR and RT-qPCR for the reliable quantification of SARS-CoV-2 RNA from stool. We identify that the Zymo DNA/RNA preservative and the QiaAMP extraction kit yield more detectable RNA than the others, using both ddPCR and RT-qPCR. Taken together, we recommend a comprehensive methodology for preservation, extraction and detection of RNA from SARS-CoV-2 and other coronaviruses in stool.
Search related documents:
Co phrase search for related documents- absolute quantification and accurate detection: 1, 2
- absolute quantification and accurate quantification: 1, 2, 3, 4
- absolute quantification and acid detection: 1, 2, 3, 4, 5, 6
- absolute quantification and acute respiratory syndrome: 1, 2, 3, 4, 5, 6, 7, 8, 9
- accessible platform and acute respiratory syndrome: 1, 2
- accurate detection and acid detection: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- accurate detection and acute respiratory syndrome: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- accurate quantification and acute respiratory syndrome: 1, 2, 3, 4, 5, 6, 7
- acid detection and acute respiratory syndrome: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- acid detection and adequate number: 1
- acid extraction kit and acute respiratory syndrome: 1, 2, 3, 4
- acute respiratory syndrome and adequate number: 1, 2, 3, 4
Co phrase search for related documents, hyperlinks ordered by date