Author: Ben-Ami, Roni; Klochendler, Agnes; Seidel, Matan; Sido, Tal; Gurel-Gurevich, Ori; Yassour, Moran; Meshorer, Eran; Benedek, Gil; Fogel, Irit; Oiknine-Djian, Esther; Gertler, Asaf; Rotstein, Zeev; Lavi, Bruno; Dor, Yuval; Wolf, Dana G; Salton, Maayan; Drier, Yotam
Title: Pooled RNA extraction and PCR assay for efficient SARS-CoV-2 detection Cord-id: 5s03f0pp Document date: 2020_4_22
ID: 5s03f0pp
Snippet: Testing for active SARS-CoV-2 infection is a fundamental tool in public health measures taken to control the COVID-19 pandemic. Due to the overwhelming use of SARS-CoV-2 RT-PCR tests worldwide, availability of test kits has become a major bottleneck. Here we demonstrate the reliability and efficiency of two simple pooling strategies that can increase testing capacity about 5-fold to 7.5-fold, in populations with a low infection rate. We have implemented the method in a routine clinical diagnosis
Document: Testing for active SARS-CoV-2 infection is a fundamental tool in public health measures taken to control the COVID-19 pandemic. Due to the overwhelming use of SARS-CoV-2 RT-PCR tests worldwide, availability of test kits has become a major bottleneck. Here we demonstrate the reliability and efficiency of two simple pooling strategies that can increase testing capacity about 5-fold to 7.5-fold, in populations with a low infection rate. We have implemented the method in a routine clinical diagnosis setting, and already tested 2,168 individuals for SARS-CoV-2 using 311 RNA extraction and RT-PCR kits.
Search related documents:
Co phrase search for related documents- accessible reliable and active sars infection accessible reliable diagnosis: 1
- accessible reliable diagnosis and active sars infection: 1
- accessible reliable diagnosis and active sars infection accessible reliable diagnosis: 1
- additional confidence and low risk profile: 1
- additional confidence and low risk profile negative: 1
- additional confidence and low risk profile negative likely: 1
- lysate preparation and lysis buffer: 1
Co phrase search for related documents, hyperlinks ordered by date