Selected article for: "Ribo seq seq and RNA seq"
Author: Hazel Stewart; Katherine Brown; Adam M. Dinan; Nerea Irigoyen; Eric J. Snijder; Andrew E. Firth
Title: The transcriptional and translational landscape of equine torovirus
  • Document date: 2018_4_7
    • ID: mozfm5ds_12
    Snippet: Tandem RNA-seq and Ribo-seq of EToV infected cells. We conducted tandem RNA-132 seq and Ribo-seq of EToV infected equine dermal (ED) cells. Two biological replicates 133 of virus-infected and mock-infected cells were analysed, generating 25 to 53 million 134 reads per sample. For RNA-seq, 77-92 % of reads mapped to the host genome, of 135 which a mean of 1.5 % mapped to rRNA, 19 % to mRNA, 32 % to ncRNA and 47 % 136 elsewhere in the genome. For R.....
    Document: Tandem RNA-seq and Ribo-seq of EToV infected cells. We conducted tandem RNA-132 seq and Ribo-seq of EToV infected equine dermal (ED) cells. Two biological replicates 133 of virus-infected and mock-infected cells were analysed, generating 25 to 53 million 134 reads per sample. For RNA-seq, 77-92 % of reads mapped to the host genome, of 135 which a mean of 1.5 % mapped to rRNA, 19 % to mRNA, 32 % to ncRNA and 47 % 136 elsewhere in the genome. For Ribo-seq, 46-60 % of reads mapped to the host 137 genome, of which a mean of 56 % mapped to rRNA, 13 % to mRNA, 4.9 % to ncRNA 138 and 26 % elsewhere in the genome (Supplementary Table 1 ). 1.3 % and 2.3 % of 139 reads mapped to the virus genome in the two EToV-infected RNA-seq replicates and 140 0.41 % and 0.21 % in the two virus-infected Ribo-seq replicates. 141

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