Author: Aravinth Kumar Jayabalan; Diane E. Griffin; Anthony K. L. Leung
Title: Alphavirus nsP3 ADP-ribosylhydrolase Activity Disrupts Stress Granule Formation Document date: 2019_6_20
ID: n8sjpcbs_46
Snippet: Immunoblot analysis: Cells were lysed in RIPA buffer (50 mM Tris-Cl pH 8.0, 150 mM NaCl, 0.1% SDS, 1% NP-40, 1 mM EDTA, containing proteinase inhibitors 5 mM NaF, 1 mM PMSF) for 15 min on ice followed by centrifugation at high speed for 15 min, 4 o C. Protein samples were acetone-precipitated All rights reserved. No reuse allowed without permission......
Document: Immunoblot analysis: Cells were lysed in RIPA buffer (50 mM Tris-Cl pH 8.0, 150 mM NaCl, 0.1% SDS, 1% NP-40, 1 mM EDTA, containing proteinase inhibitors 5 mM NaF, 1 mM PMSF) for 15 min on ice followed by centrifugation at high speed for 15 min, 4 o C. Protein samples were acetone-precipitated All rights reserved. No reuse allowed without permission.
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