Author: Dupas Enora; Briand Martial; Jacques Marie-Agnès; Cesbron Sophie
Title: Novel tetraplex qPCR assays for simultaneous detection and identification of Xylella fastidiosa subspecies in plant tissues Document date: 2019_7_11
ID: 65qpcsiq_57
Snippet: improve per se the DNA extraction step and improve the LOD of our assays. Various options are 463 available. A phenol-chloroform step could be added to the DNA extraction method to reduce the level 464 of extracted proteins (Schrader et al., 2012) . Reagents such as Tween 20, DMSO, polyethylene glycol 465 or active carbon could be used to precipitate the polysaccharides before DNA precipitation (Schrader 466 et al., 2012) . Phenol levels may be r.....
Document: improve per se the DNA extraction step and improve the LOD of our assays. Various options are 463 available. A phenol-chloroform step could be added to the DNA extraction method to reduce the level 464 of extracted proteins (Schrader et al., 2012) . Reagents such as Tween 20, DMSO, polyethylene glycol 465 or active carbon could be used to precipitate the polysaccharides before DNA precipitation (Schrader 466 et al., 2012) . Phenol levels may be reduced with the use of polyvinyl-pyrrolidone or the addition of 467 . CC-BY-NC-ND 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/699371 doi: bioRxiv preprint borate (Wilkins and Smart, 1996) . Drying plant samples at 65°C for 2 days, prior to DNA extraction, 468
Search related documents:
Co phrase search for related documents- cc NC ND International license and extraction step: 1
- cc NC ND International license and International license: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- cc NC ND International license and phenol chloroform: 1
- dna extraction and extract protein: 1
- dna extraction and extraction method: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24
- dna extraction and extraction step: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
- dna extraction and International license: 1, 2, 3, 4, 5
- dna extraction and phenol chloroform: 1, 2, 3, 4, 5, 6, 7, 8
- dna extraction method and extraction method: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15
- dna extraction method and extraction step: 1
- dna extraction method and phenol chloroform: 1
- dna extraction step and extraction method: 1
- dna extraction step and extraction step: 1, 2, 3, 4, 5, 6, 7, 8, 9
- dna precipitation and extraction method: 1
- dna precipitation and phenol chloroform: 1, 2
- extract protein and International license: 1, 2
- extraction method and phenol chloroform: 1, 2, 3, 4
- extraction step and International license: 1, 2
- International license and phenol chloroform: 1
Co phrase search for related documents, hyperlinks ordered by date