Porcine epidemic diarrhea virus virus-like particles produced in insect cells induce specific immune responses in mice

Wang, Cuiling; Yan, Feihu; Zheng, Xuexing; Wang, Hualei; Jin, Hongli; Wang, Chong; Zhao, Yongkun; Feng, Na; Wang, Tiecheng; Gao, Yuwei; Yang, Songtao; Xia, Xianzhu

Porcine epidemic diarrhea virus (PEDV), which causes 80–100% mortality in neonatal piglets, is one of the most devastating viral diseases affecting swine worldwide. To date, the lack of effective vaccines and drugs is the main problem preventing control of the global spread of PEDV. In this study, we produced PEDV virus-like particles (VLPs) composed of S, M, and E proteins with a baculovirus expression system and tested them via indirect immunofluorescence assay (IFA)and Western blot analysis. Electron microscopy showed that the morphological structure of the PEDV VLPs was similar to that of the protovirus. Microneutralization assays and ELISpot analysis demonstrated that PEDV VLPs induced highly specific antibody responses and Th2-mediated humoral immunity. As a result, the PEDV VLPs displayed excellent immunogenicity in mice. Therefore, a VLP-based vaccine has the potential to prevent PEDV infection.