Author: Martina F Scallan; Catherine Dempsey; John MacSharry; Isabelle O'Callaghan; Paula M O'Connor; Conor P Hogan; Edel Durack; Paul D. Cotter; Sarah Hudson; Humphrey A Moynihan; Brigid Lucey
Title: Validation of a Lysis Buffer Containing 4 M Guanidinium Thiocyanate (GITC)/ Triton X-100 for Extraction of SARS-CoV-2 RNA for COVID-19 Testing: Comparison of Formulated Lysis Buffers Containing 4 to 6 M GITC, Roche External Lysis Buffer and Qiagen RTL Lysis Buffer Document date: 2020_4_8
ID: jpb9a8c4_16
Snippet: The validation consisted of a comparison of the efficacy of extraction with four laboratoryprepared buffers and both a Qiagen (RLT lysis buffer from an RNeasy kit Cat. No./ID: 79216) and a Roche external lysis buffer when testing positive (amplifying two Covid-19 specific targets) and negative controls......
Document: The validation consisted of a comparison of the efficacy of extraction with four laboratoryprepared buffers and both a Qiagen (RLT lysis buffer from an RNeasy kit Cat. No./ID: 79216) and a Roche external lysis buffer when testing positive (amplifying two Covid-19 specific targets) and negative controls.
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