Author: Martina F Scallan; Catherine Dempsey; John MacSharry; Isabelle O'Callaghan; Paula M O'Connor; Conor P Hogan; Edel Durack; Paul D. Cotter; Sarah Hudson; Humphrey A Moynihan; Brigid Lucey
Title: Validation of a Lysis Buffer Containing 4 M Guanidinium Thiocyanate (GITC)/ Triton X-100 for Extraction of SARS-CoV-2 RNA for COVID-19 Testing: Comparison of Formulated Lysis Buffers Containing 4 to 6 M GITC, Roche External Lysis Buffer and Qiagen RTL Lysis Buffer Document date: 2020_4_8
ID: jpb9a8c4_6
Snippet: The 4 M GITC lysis buffer was developed based on knowledge of the role of GITC in RNA extraction and protection (Chomczynski & Sacchi, 1987; Boom et al., 1990) , adapted for magnetic glass bead extraction (Hui He et al., 2017) , and validated against recognised standards using positive and negative controls. Details of the composition of 4 M GITC lysis buffer and for the preparation of 1 litre are given below......
Document: The 4 M GITC lysis buffer was developed based on knowledge of the role of GITC in RNA extraction and protection (Chomczynski & Sacchi, 1987; Boom et al., 1990) , adapted for magnetic glass bead extraction (Hui He et al., 2017) , and validated against recognised standards using positive and negative controls. Details of the composition of 4 M GITC lysis buffer and for the preparation of 1 litre are given below.
Search related documents:
Co phrase search for related documents- GITC lysis buffer and RNA extraction: 1
- glass bead and lysis buffer: 1
- glass bead and RNA extraction: 1, 2
- glass bead extraction and lysis buffer: 1
- glass bead extraction and RNA extraction: 1, 2
- lysis buffer and protection RNA extraction: 1
- lysis buffer and RNA extraction: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- negative positive control and RNA extraction: 1, 2, 3, 4, 5
- protection RNA extraction and RNA extraction: 1
Co phrase search for related documents, hyperlinks ordered by date