Selected article for: "extraction kit and internal control"
Author: jerome lechien; pierre cabaraux; carlos chiesa-estomba; mohammad khalife; jan plzak; stephane hans; delphine martiny; christian Henriquez; Claire hopkins; sven saussez
Title: Objective olfactory testing in patients presenting with sudden onset olfactory dysfunction as the first manifestation of confirmed COVID-19 infection
  • Document date: 2020_4_18
    • ID: l2cc6wd8_19
    Snippet: (which was not peer-reviewed) The copyright holder for this preprint . https://doi.org/10.1101/2020.04.15.20066472 doi: medRxiv preprint confirmation of SARS-CoV-2 was performed by reverse transcription polymerase chain reaction (RT-PCR) assays. Viral RNA extraction was performed by m2000 mSample Preparation SystemDNA Kit (Abbott) using 1000µl manually lysed sample (700µl sample + 800µl lysis buffer from kit) eluted in 90µl elution buffer. A .....
    Document: (which was not peer-reviewed) The copyright holder for this preprint . https://doi.org/10.1101/2020.04.15.20066472 doi: medRxiv preprint confirmation of SARS-CoV-2 was performed by reverse transcription polymerase chain reaction (RT-PCR) assays. Viral RNA extraction was performed by m2000 mSample Preparation SystemDNA Kit (Abbott) using 1000µl manually lysed sample (700µl sample + 800µl lysis buffer from kit) eluted in 90µl elution buffer. A qRT-PCR internal control was added at each extraction. qRT-PCR was performed using 10µl of extracted sample in the RealStar ® SARS-CoV-2 RT-PCR Kit from altona-diagnostics with a cut-off set at 40 Ct.

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