Selected article for: "guanidinium thiocyanate and lysis buffer"
Author: Martina F Scallan; Catherine Dempsey; John MacSharry; Isabelle O'Callaghan; Paula M O'Connor; Conor P Hogan; Edel Durack; Paul D. Cotter; Sarah Hudson; Humphrey A Moynihan; Brigid Lucey
Title: Validation of a Lysis Buffer Containing 4 M Guanidinium Thiocyanate (GITC)/ Triton X-100 for Extraction of SARS-CoV-2 RNA for COVID-19 Testing: Comparison of Formulated Lysis Buffers Containing 4 to 6 M GITC, Roche External Lysis Buffer and Qiagen RTL Lysis Buffer
  • Document date: 2020_4_8
    • ID: jpb9a8c4_22
    Snippet: A 4 M GITC lysis buffer with 3 % (v/v) Triton X-100 has been shown to work very well in COVID-19 RT-PCR, it includes a detergent which helps to disintegrate the virus during extraction, bromophenol blue as a visual aid for addition of lysis buffer to clinical samples and has the advantage of using less of a scarce ingredient. The knowledge that 4 M guanidinium thiocyanate (GITC) is sufficient in this lysis buffer means the straitened global suppl.....
    Document: A 4 M GITC lysis buffer with 3 % (v/v) Triton X-100 has been shown to work very well in COVID-19 RT-PCR, it includes a detergent which helps to disintegrate the virus during extraction, bromophenol blue as a visual aid for addition of lysis buffer to clinical samples and has the advantage of using less of a scarce ingredient. The knowledge that 4 M guanidinium thiocyanate (GITC) is sufficient in this lysis buffer means the straitened global supply of this chemical can be shared more effectively to make more lysis buffer to perform more tests around the world and help the world to take back control against this pandemic virus.

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