The permeabilized SecY protein-translocation channel can serve as a nonspecific sugar transporter

Sen Mei; Chong Xie; Hao Mi; Chuang Xue; Qiang Guo; Guang-Qing Du; Guo-Bei Li; Cai-Xia Li; Ya-Nan Qu; Ming-Hao Xiong; Yang Jiang; Tian-Wei Tan; Shang-Tian Yang; Li-Hai Fan

The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/378786 doi: bioRxiv preprint 7 that the fructose taken up via the fructose PTS is concomitantly phosphorylated to 125 fructose-1-phosphate before further phosphorylation to fructose-1,6-bisphosphate 126 (Kornberg, 2001) (Fig 1A) , but in our route, the phosphorylated fructose first appears 127 inside the cell as fructose-6-phosphate (Fig 1C) , which is in agreement with the route 128 using the transporter of PtsG-F (Kornberg et al, 2000) . The data of growth kinetics in when compared with the wild-type E. coli (Fig 3G and J) , which was also found in the 143 case of mannose ( Fig 3D) . Interestingly, previous work reported that a mutant glucose 144 facilitator protein (2-RD5) from Zymomonas mobilis allowed rapid transport of xylose in 145 E. coli, but in contrast, 2-RD5 caused an extensive growth delay (Ren et al, 2009) . A 146 prolonged lag phase (≈ 25 h) on arabinose was also observed when we re-expressed 147 All rights reserved. No reuse allowed without permission.