Selected article for: "affinity chromatography and inclusion body"
Author: Amrita Roy; Liangzhong Lim; Shagun Srivastava; Jianxing Song
Title: Unique properties of Zika NS2B-NS3pro complexes as decoded by experiments and MD simulations
  • Document date: 2016_9_28
    • ID: l82aakrk_10
    Snippet: We also constructed a Zika protease with NS2B and NS3pro linked by a (Gly)4-Ser-(Gly)4 sequence extensively used for functional and structural characterization of flavivirus NS2B-NS3pro complexes (13) (14) (15) (16) (17) (18) (19) (20) . No linked NS2B-NS3pro protein was detected in the supernatant, but we were able to purify a small amount from the inclusion body by the Ni 2+affinity chromatography under the denaturing condition, which was confi.....
    Document: We also constructed a Zika protease with NS2B and NS3pro linked by a (Gly)4-Ser-(Gly)4 sequence extensively used for functional and structural characterization of flavivirus NS2B-NS3pro complexes (13) (14) (15) (16) (17) (18) (19) (20) . No linked NS2B-NS3pro protein was detected in the supernatant, but we were able to purify a small amount from the inclusion body by the Ni 2+affinity chromatography under the denaturing condition, which was confirmed to be the linked NS2B-NS3pro by sequencing with mass spectrometry and western plot with the His-tag antibody. Linked NS2B-NS3pro was successfully refolded by dialysis without any author/funder. All rights reserved. No reuse allowed without permission.

    Search related documents:
    Co phrase search for related documents
    • mass spectrometry and Ni chromatography: 1, 2, 3
    • Ni chromatography and tag antibody: 1