Author: Amrita Roy; Liangzhong Lim; Shagun Srivastava; Jianxing Song
Title: Unique properties of Zika NS2B-NS3pro complexes as decoded by experiments and MD simulations Document date: 2016_9_28
ID: l82aakrk_54
Snippet: Fluorescence, CD and NMR experiments-Intrinsic UV fluorescence spectra were measured with a Cary Eclipse fluorescence spectrophotometer as we previously described (24) with the excitation wavelength at 280 nm. Circular dichroism (CD) experiments were performed on a Jasco J-1500 spectropolarimeter and data from five independent scans were added and averaged (12, 24) . All NMR experiments were acquired on an 800 MHz Bruker Avance spectrometer equip.....
Document: Fluorescence, CD and NMR experiments-Intrinsic UV fluorescence spectra were measured with a Cary Eclipse fluorescence spectrophotometer as we previously described (24) with the excitation wavelength at 280 nm. Circular dichroism (CD) experiments were performed on a Jasco J-1500 spectropolarimeter and data from five independent scans were added and averaged (12, 24) . All NMR experiments were acquired on an 800 MHz Bruker Avance spectrometer equipped with pulse field gradient units as described previously (12, 24, 36) . For steady state kinetics, we used the exactly the same buffer as a previous one on profiling substrate specificity for the NS2B-NS3pro complexes of all four Dengue serotypes (17) : 50 mM Tris-HCl at pH 8.5, except that no glycerol was added as it would lead to significant NMR signal broadening due to high viscosity. Briefly, Zika protease at 50 nM was author/funder. All rights reserved. No reuse allowed without permission.
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