Author: Deguo Wang
Title: One-pot Detection of COVID-19 with Real-time Reverse-transcription Loop-mediated Isothermal Amplification (RT-LAMP) Assay and Visual RT-LAMP Assay Document date: 2020_4_22
ID: krim73zt_7
Snippet: The effect of inhibitors in blood samples on the amplification efficiency had been determined, as Figure 2 indicated, when the real-time RT-LAMP reaction system was added with 10 µL blood samples dissolved in 10 µL 2019-nCoV-Fast-Sample Nucleic Acid Releasing Agent (Hunan Shengxiang Biology Technology Co., Ltd, China), there was no amplification; when 7.5 µL blood samples and 10 µL 2019-nCoV-Fast-Sample Nucleic Acid Releasing Agent added, the.....
Document: The effect of inhibitors in blood samples on the amplification efficiency had been determined, as Figure 2 indicated, when the real-time RT-LAMP reaction system was added with 10 µL blood samples dissolved in 10 µL 2019-nCoV-Fast-Sample Nucleic Acid Releasing Agent (Hunan Shengxiang Biology Technology Co., Ltd, China), there was no amplification; when 7.5 µL blood samples and 10 µL 2019-nCoV-Fast-Sample Nucleic Acid Releasing Agent added, there was slight amplification; when 5µL or 2.5 µL blood samples dissolved in 10 µL 2019-nCoV-Fast-Sample Nucleic Acid Releasing Agent added, there was acceptable effect on the real-time RT-LAMP reaction. Therefore, the blood sample volume of 5µL was selected for 50 µL one-pot real-time or visual RT-LAMP reaction.
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