Selected article for: "multiplex reaction mix and reaction mix"
Author: Monica Sentmanat; Evguenia Kouranova; Xiaoxia Cui
Title: One-step RNA extraction for RT-qPCR detection of 2019-nCoV
  • Document date: 2020_4_5
    • ID: ihhu7nef_19
    Snippet: Probe and primer sets were obtained from the research COVID19 RT-qPCR kits 2019-nCoV RUO (IDT Cat. No. 10006713). A one-step reaction mix was prepared using Reliance One-Step Multiplex Supermix (BioRad Cat. No. 12010220) with 2 ul of sample, 1.5 ul of the probe/prime set mix for each reaction, 5 ul of 4x reaction mix, and 11.5 ul of molecular biology grade water to a final volume of 20 ul. The RT-qPCR was run on a QuantStudioâ„¢ 3 real-time PCR m.....
    Document: Probe and primer sets were obtained from the research COVID19 RT-qPCR kits 2019-nCoV RUO (IDT Cat. No. 10006713). A one-step reaction mix was prepared using Reliance One-Step Multiplex Supermix (BioRad Cat. No. 12010220) with 2 ul of sample, 1.5 ul of the probe/prime set mix for each reaction, 5 ul of 4x reaction mix, and 11.5 ul of molecular biology grade water to a final volume of 20 ul. The RT-qPCR was run on a QuantStudioâ„¢ 3 real-time PCR machine (ThermoFisher).

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