Author: Feria Hikmet; Loren Méar; Mathias Uhlén; Cecilia Lindskog
Title: The protein expression profile of ACE2 in human tissues Document date: 2020_4_3
ID: 4fz6iqwy_29
Snippet: Data analysis including filtering, normalization, and clustering were performed using Seurat V3.0 (https://satijalab.org/seurat/9) in R (CRAN). Cells were removed when they had less than 500 genes and greater than 20% reads mapped to mitochondrial expression genome. The gene expression data were normalized using default settings. Cell clustering was based on the 5000 highly variable genes. All scatter plots were obtained using the UMAP method. Cl.....
Document: Data analysis including filtering, normalization, and clustering were performed using Seurat V3.0 (https://satijalab.org/seurat/9) in R (CRAN). Cells were removed when they had less than 500 genes and greater than 20% reads mapped to mitochondrial expression genome. The gene expression data were normalized using default settings. Cell clustering was based on the 5000 highly variable genes. All scatter plots were obtained using the UMAP method. Cluster identity was manually assigned based on well-known cell-type markers and named based on the main cell type in each cluster.
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