Selected article for: "host factor and protein domain"

Author: K. Reddisiva Prasanth; Minato Hirano; W. Samuel Fagg; Eileen T. McAnarney; Chao Shan; Xuping Xie; Adam Hage; Colette A. Pietzsch; Alexander Bukreyev; Ricardo Rajsbaum; Pei-Yong Shi; Mark T. Bedford; Shelton S. Bradrick; Vineet Menachery; Mariano A. Garcia-Blanco
Title: Topoisomerase III-ß is required for efficient replication of positive-sense RNA viruses
  • Document date: 2020_3_27
  • ID: a0xfb9l4_3
    Snippet: A meta-analysis of RNAi-based loss-of-function screens for YFV and DENV-2 host factors revealed 274 common candidates 1 . TDRD3, a Tudor domain containing protein that interacts with methylated arginine motifs 5 , was identified as a candidate host factor required for both DENV2/YFV. In the two YFV screens TDRD3 ranked 66 th of over 21,500 gene products in terms of how well its knockdown decreased YFV (adjusted p value = 0.0006). CRISPR-Cas9 medi.....
    Document: A meta-analysis of RNAi-based loss-of-function screens for YFV and DENV-2 host factors revealed 274 common candidates 1 . TDRD3, a Tudor domain containing protein that interacts with methylated arginine motifs 5 , was identified as a candidate host factor required for both DENV2/YFV. In the two YFV screens TDRD3 ranked 66 th of over 21,500 gene products in terms of how well its knockdown decreased YFV (adjusted p value = 0.0006). CRISPR-Cas9 mediated knockout of TDRD3 in HuH-7 cells confirmed that this protein was required for efficient DENV-2 replication (Fig 1A & B) .

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