Author: Beaudoin-Bussières, Guillaume; Richard, Jonathan; Prévost, Jérémie; Goyette, Guillaume; Finzi, Andrés
Title: A new flow cytometry assay to measure antibody dependent cellular cytotoxicity against SARS-CoV-2 Spike expressing cells Cord-id: ip5u2lgm Document date: 2021_9_13
ID: ip5u2lgm
Snippet: Antibodies can engage specific receptors at the surface of effector cells and mediate several functions beyond viral neutralization. Increasing evidence suggest that Fc-mediated effector functions, such as antibody dependent cellular cytotoxicity (ADCC), have an important role in protection against SARS-CoV-2 infection. We engineered a cell line stably expressing a GFP-tagged SARS-CoV-2 Spike to measure ADCC. This protocol provides an optimized way of measuring ADCC activity mediated by anti-SAR
Document: Antibodies can engage specific receptors at the surface of effector cells and mediate several functions beyond viral neutralization. Increasing evidence suggest that Fc-mediated effector functions, such as antibody dependent cellular cytotoxicity (ADCC), have an important role in protection against SARS-CoV-2 infection. We engineered a cell line stably expressing a GFP-tagged SARS-CoV-2 Spike to measure ADCC. This protocol provides an optimized way of measuring ADCC activity mediated by anti-SARS-CoV-2 Spike monoclonal antibodies or plasma from previously infected or vaccinated individuals.
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