Author: Siridechadilok, Bunpote; Gomutsukhavadee, Methee; Sawaengpol, Thunyarat; Sangiambut, Sutha; Puttikhunt, Chunya; Chin-inmanu, Kwanrutai; Suriyaphol, Prapat; Malasit, Prida; Screaton, Gavin; Mongkolsapaya, Juthathip
Title: A Simplified Positive-Sense-RNA Virus Construction Approach That Enhances Analysis Throughput Cord-id: 84gvwb35 Document date: 2013_12_25
ID: 84gvwb35
Snippet: Here we present an approach that advances the throughput of a genetic analysis of a positive-sense RNA virus by simplifying virus construction. It enabled comprehensive dissection of a complex, multigene phenotype through rapid derivation of a large number of chimeric viruses and construction of a mutant library directly from a virus pool. The versatility of the approach described here expands the applicability of diverse genetic approaches to study these viruses.
Document: Here we present an approach that advances the throughput of a genetic analysis of a positive-sense RNA virus by simplifying virus construction. It enabled comprehensive dissection of a complex, multigene phenotype through rapid derivation of a large number of chimeric viruses and construction of a mutant library directly from a virus pool. The versatility of the approach described here expands the applicability of diverse genetic approaches to study these viruses.
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