Author: Danielle E Anderson; Jin Cui; Qian Ye; Baoying Huang; Wenhong Zu; Jing Gong; Weiqiang Liu; So Young Kim; Biao Guo Yan; Kristmundur Sigmundsson; Xiao Fang Lim; Fei Ye; Peihua Niu; Xuming Zhou; Wenjie Tan; Lin-Fa Wang; Xu Tan
Title: Orthogonal genome-wide screenings in bat cells identify MTHFD1 as a target of broad antiviral therapy Document date: 2020_3_30
ID: 0l33i6s4_62
Snippet: Virus infected cells were fixed with 4% paraformaldehyde (PFA) for 10min at room 9 temperature (RT), and permeated with 0.2% Triton X-100 for another 10min at RT, the cells were 10 washed with PBS for 3 times and incubated with PR8 HA antibody or ZIKV E protein antibody 11 for 2h at RT or 4℃ overnight, then washed with PBS for 3 times and incubated with second 12 antibody AF488 (goat anti mouse). The nuclei were stained with DAPI. Images were a.....
Document: Virus infected cells were fixed with 4% paraformaldehyde (PFA) for 10min at room 9 temperature (RT), and permeated with 0.2% Triton X-100 for another 10min at RT, the cells were 10 washed with PBS for 3 times and incubated with PR8 HA antibody or ZIKV E protein antibody 11 for 2h at RT or 4℃ overnight, then washed with PBS for 3 times and incubated with second 12 antibody AF488 (goat anti mouse). The nuclei were stained with DAPI. Images were acquired 13 with Cellomic ArrayScan VTI HCS (Thermo Scientific) 14 15
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