Selected article for: "liquid nitrogen and OCT compound"

Author: Takako I. Jones; Guo-Liang Chew; Pamela Barraza-Flores; Spencer Schreier; Monique Ramirez; Ryan D. Wuebbles; Dean J. Burkin; Robert K. Bradley; Peter L. Jones
Title: Transgenic mice expressing tunable levels of DUX4 develop characteristic facioscapulohumeral muscular dystrophy-like pathophysiology ranging in severity
  • Document date: 2018_11_15
  • ID: 1yto01tr_15
    Snippet: Freshly dissected muscles were kept moist, coated with O.C.T. compound, snap-frozen in liquid nitrogen-cooled isopentane, and stored at -80ºC until sectioning. 12μm cryosections were mounted on slides and air-dried before staining or storage. Sections were used for H&E staining [41] or picrosirius red staining......
    Document: Freshly dissected muscles were kept moist, coated with O.C.T. compound, snap-frozen in liquid nitrogen-cooled isopentane, and stored at -80ºC until sectioning. 12μm cryosections were mounted on slides and air-dried before staining or storage. Sections were used for H&E staining [41] or picrosirius red staining.

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