Author: Chi, X. Sherry; Li, Fenglan; Tam, John S.; Rappaport, Ruth; Cheng, Sheau-Mei
Title: Semiquantitative one-step RT-PCR for simultaneous identification of human influenza and respiratory syncytial viruses Cord-id: hj1k1jtu Document date: 2006_10_9
ID: hj1k1jtu
Snippet: A multiplex reverse transcription-polymerase chain reaction (mRT-PCR) method was developed for simultaneous detection and typing/subtyping of influenza viruses A/H1, A/H3 or B, and respiratory syncytial viruses A or B, followed by DNA semiquantitation using the Agilent 2100 Bioanalyzer. Such method provides a rapid, specific and sensitive diagnostic tool for detection and semiquantification of respiratory illness specimens.
Document: A multiplex reverse transcription-polymerase chain reaction (mRT-PCR) method was developed for simultaneous detection and typing/subtyping of influenza viruses A/H1, A/H3 or B, and respiratory syncytial viruses A or B, followed by DNA semiquantitation using the Agilent 2100 Bioanalyzer. Such method provides a rapid, specific and sensitive diagnostic tool for detection and semiquantification of respiratory illness specimens.
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