Author: Paul A. Rowley; Brandon Ho; Sarah Bushong; Arlen Johnson; Sara L. Sawyer
Title: XRN1 is a Species-Specific Virus Restriction Factor in Yeasts Document date: 2016_8_16
ID: 1znuxoj5_57
Snippet: The curing of the killer phenotype was measured by transforming S. cerevisiae BJH006 804 with approximately 100 ng of plasmid encoding various XRN1 genes using the LiAc 805 method. The addition of 1000 ng or as little as 10 ng of plasmid had no affect on the 806 percentage of colonies cured using this assay. After 48 h of growth, colonies were 807 streaked out and grown for a further 48 h. Clonal isolates of killer yeasts were patched 808 onto a .....
Document: The curing of the killer phenotype was measured by transforming S. cerevisiae BJH006 804 with approximately 100 ng of plasmid encoding various XRN1 genes using the LiAc 805 method. The addition of 1000 ng or as little as 10 ng of plasmid had no affect on the 806 percentage of colonies cured using this assay. After 48 h of growth, colonies were 807 streaked out and grown for a further 48 h. Clonal isolates of killer yeasts were patched 808 onto a YPD "killer assay" plate (see kill zone measurement protocol) that were 809 previously inoculated with S. cerevisiae K12, and incubated at room temperature for 72 810 h. The presence or absence of a zone of inhibition was used to calculate the percentage 811 of killer yeast clones cured of the killer phenotype.
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