Author: Martina F Scallan; Catherine Dempsey; John MacSharry; Isabelle O'Callaghan; Paula M O'Connor; Conor P Hogan; Edel Durack; Paul D. Cotter; Sarah Hudson; Humphrey A Moynihan; Brigid Lucey
Title: Validation of a Lysis Buffer Containing 4 M Guanidinium Thiocyanate (GITC)/ Triton X-100 for Extraction of SARS-CoV-2 RNA for COVID-19 Testing: Comparison of Formulated Lysis Buffers Containing 4 to 6 M GITC, Roche External Lysis Buffer and Qiagen RTL Lysis Buffer Document date: 2020_4_8
ID: jpb9a8c4_2
Snippet: Expansion of PCR testing is critical to gain control over the pandemic spread of COVID-19 and the world-wide shortage of lysis buffer is a rate limiting step. Here we disseminate details of a lysis buffer formulation that came into existence as a consequence of a rapidly formed multi-centre collaboration. A replacement lysis buffer for extraction of viral nucleic acids from respiratory samples was desperately needed by diagnostic laboratories. A .....
Document: Expansion of PCR testing is critical to gain control over the pandemic spread of COVID-19 and the world-wide shortage of lysis buffer is a rate limiting step. Here we disseminate details of a lysis buffer formulation that came into existence as a consequence of a rapidly formed multi-centre collaboration. A replacement lysis buffer for extraction of viral nucleic acids from respiratory samples was desperately needed by diagnostic laboratories. A panel of new lysis buffer formulations was generated and validated for use in COVID-19 testing by virus-specific RT-PCR and are being distributed throughout Ireland. It is hoped that by sharing the formulation and validation, we can make it useful to others in the national and international scientific community.
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