Author: Martina F Scallan; Catherine Dempsey; John MacSharry; Isabelle O'Callaghan; Paula M O'Connor; Conor P Hogan; Edel Durack; Paul D. Cotter; Sarah Hudson; Humphrey A Moynihan; Brigid Lucey
Title: Validation of a Lysis Buffer Containing 4 M Guanidinium Thiocyanate (GITC)/ Triton X-100 for Extraction of SARS-CoV-2 RNA for COVID-19 Testing: Comparison of Formulated Lysis Buffers Containing 4 to 6 M GITC, Roche External Lysis Buffer and Qiagen RTL Lysis Buffer Document date: 2020_4_8
ID: jpb9a8c4_7
Snippet: The lysis procedure used in the diagnostic laboratory mixes lysis buffer and sample 1:1, this generates a working concentration of 2 M GITC during the lysis step as reported to be optimal by Hui He et al., 2017......
Document: The lysis procedure used in the diagnostic laboratory mixes lysis buffer and sample 1:1, this generates a working concentration of 2 M GITC during the lysis step as reported to be optimal by Hui He et al., 2017.
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