Author: Ebru Oral; Keith K Wannomae; Rachel L Connolly; Joseph A Gardecki; Hui Min Leung; Orhun K Muratoglu; Anthony Griffiths; Anna N Honko; Laura E Avena; Lindsay GA McKay; Nick Flynn; Nadia Storm; Sierra N Downs; Ralph Jones; Brandon Emmal
Title: Vapor H2O2 sterilization as a decontamination method for the reuse of N95 respirators in the COVID-19 emergency Document date: 2020_4_16
ID: gty8pxkz_10
Snippet: SARS-CoV-2 USA-WA1/2020 [1] was propagated in Vero E6 cells (BEI resources, NIAID, NIH: VERO C1008 (E6), African green monkey kidney, Working Bank #NR-596). Virus stock used for these studies had been passaged thrice in VERO cells and twice in VeroE6 cells. Cells were maintained in Dulbecco's modified Eagle medium (DMEM) with GlutaMAXâ„¢ and sodium pyruvate (Gibcoâ„¢, ThermoFisher Scientific, Waltham, MA, catalog 10569), non-essential amino acids.....
Document: SARS-CoV-2 USA-WA1/2020 [1] was propagated in Vero E6 cells (BEI resources, NIAID, NIH: VERO C1008 (E6), African green monkey kidney, Working Bank #NR-596). Virus stock used for these studies had been passaged thrice in VERO cells and twice in VeroE6 cells. Cells were maintained in Dulbecco's modified Eagle medium (DMEM) with GlutaMAXâ„¢ and sodium pyruvate (Gibcoâ„¢, ThermoFisher Scientific, Waltham, MA, catalog 10569), non-essential amino acids (Gibcoâ„¢, catalog 11140) and 10% HI-FBS (Gibcoâ„¢, catalog 10082).
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