Clinical factors associated with composition of lung microbiota and important taxa predicting clinical prognosis in patients with severe community-acquired pneumonia

Du, Sisi; Wu, Xiaojing; Li, Binbin; Wang, Yimin; Shang, Lianhan; Huang, Xu; Xia, Yudi; Yu, Donghao; Lu, Naicong; Liu, Zhibo; Wang, Chunlei; Liu, Xinmeng; Xiong, Zhujia; Zou, Xiaohui; Lu, Binghuai; Liu, Yingmei; Zhan, Qingyuan; Cao, Bin

Few studies have described the key features and prognostic roles of lung microbiota in patients with severe community-acquired pneumonia (SCAP). We prospectively enrolled consecutive SCAP patients admitted to ICU. Bronchoscopy was performed at bedside within 48 h of ICU admission, and 16S rRNA gene sequencing was applied to the collected bronchoalveolar lavage fluid. The primary outcome was clinical improvements defined as a decrease of 2 categories and above on a 7-category ordinal scale within 14 days following bronchoscopy. Sixty-seven patients were included. Multivariable permutational multivariate analysis of variance found that positive bacteria lab test results had the strongest independent association with lung microbiota (R(2) = 0.033; P = 0.018), followed by acute kidney injury (AKI; R(2) = 0.032; P = 0.011) and plasma MIP-1β level (R(2) = 0.027; P = 0.044). Random forest identified that the families Prevotellaceae, Moraxellaceae, and Staphylococcaceae were the biomarkers related to the positive bacteria lab test results. Multivariable Cox regression showed that the increase in α-diversity and the abundance of the families Prevotellaceae and Actinomycetaceae were associated with clinical improvements. The positive bacteria lab test results, AKI, and plasma MIP-1β level were associated with patients’ lung microbiota composition on ICU admission. The families Prevotellaceae and Actinomycetaceae on admission predicted clinical improvements. ELECTRONIC SUPPLEMENTARY MATERIAL: Supplementary material is available in the online version of this article at 10.1007/s11684-021-0856-3 and is accessible for authorized users.