Antitumor activity of Moringa oleifera (drumstick tree) flower trypsin inhibitor (MoFTI) in sarcoma 180-bearing mice.

Leite de Siqueira Patriota, Leydianne; de Brito Marques Ramos, Dalila; Lima Amorim Dos Santos, Angela Caroline; Silva, Yasmym Araújo; Gama E Silva, Mariana; Lira Torres, Diego José; Procópio, Thamara Figueiredo; Macário de Oliveira, Alisson; Breitenbach Barroso Coelho, Luana Cassandra; Pontual, Emmanuel Viana; Nunes da Silva, Diego César; Guedes Paiva, Patrícia Maria; Barros de Lorena, Vírginia Maria; Mendes, Rosemairy Luciane; Napoleão, Thiago Henrique

The plant Moringa oleifera is used as food and medicine. M. oleifera flowers are source of protein, fiber, and antioxidants, and are used to treat inflammation and tumors. This work evaluated the antitumor activity of the M. oleifera flower trypsin inhibitor (MoFTI) in sarcoma 180-bearing mice. Swiss female mice were inoculated with sarcoma 180 cells. Seven days later, the animals were treated intraperitoneally for 1 week with daily doses of PBS (control) or MoFTI (15 or 30 mg/kg). For toxicity assessment, water and food consumption, body and organ weights, histological alterations, and blood hematological and biochemical parameters were measured. Treatment with MoFTI caused pronounced reduction (90.1%-97.9%) in tumor weight. The tumors of treated animals had a reduced number of secondary vessels and lower gauge of the primary vessels compared to the control. No significant changes were observed in water and food consumption or in body and organ weights. Histopathological analysis did not indicate damage to the liver, kidneys, and spleen. In conclusion, MoFTI showed antitumor potential, with no clear evidence of toxicity.