Ultra-Low-Cost Integrated Silicon-based Transducer for On-Site, Genetic Detection of Pathogens

Estefania Nunez-Bajo; Michael Kasimatis; Yasin Cotur; Tarek Asfour; Alex Collins; Ugur Tanriverdi; Max Grell; Matti Kaisti; Guglielmo Senesi; Karen Stevenson; Firat Guder

The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.23. Once the basic device structure was created, the wafer was laser-diced into individual chips (37 chips / 4" Si wafer, Figure 1B ). Using the Randles-Sevcik equation with experimentally measured gradient values of 0.71 ± 0.08, we determined that the Au film has a 2x larger electroactive area than the geometrically defined area due to the high porosity of the Au film, which is beneficial for highperformance electrochemical analysis ( Figure S2) . A sample reservoir (polyethylene; diameter: 5 mm; volume: 40 µl, Figure 1A ) with two holes was thermally bonded across the three-electrode electrochemical cell at 110±10 °C. The sample reservoir is specifically designed to prevent the evaporation of the solvent during amplification of DNA at elevated temperatures. The final TriSilix chip has a thickness of 490 µm (N=7) in the WE region and 650 µm in the RE/CE region (N=7) without the sample reservoir. Figure 1C shows a schematic illustration of the functional building blocks of TriSilix that provide trimodal operation: The three-electrode electrochemical cell fabricated on the top surface is connected to an electrochemical reader (potentiostat) to provide an author/funder. All rights reserved. No reuse allowed without permission.