Ultra-Low-Cost Integrated Silicon-based Transducer for On-Site, Genetic Detection of Pathogens

Estefania Nunez-Bajo; Michael Kasimatis; Yasin Cotur; Tarek Asfour; Alex Collins; Ugur Tanriverdi; Max Grell; Matti Kaisti; Guglielmo Senesi; Karen Stevenson; Firat Guder

The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.23.002931 doi: bioRxiv preprint 9 measurement (see Supporting Information Section S3 for more information on the calibration of the TriSilix thermistor). Temperature sensing is important for correct and fast execution of a NA amplification program for at least two reasons: i) Temperature can be maintained at a precise setpoint using a control algorithm such as proportional-integral-derivative control loop. ii) When cooling (passively) or heating (actively), the speed of reaching a temperature setpoint can be maximized as both heating and cooling depend on the outside temperature and device packaging (e.g., the construction of the holder or cartridge). By only relying on the power applied, temperature control cannot be achieved with high precision.