Author: Estefania Nunez-Bajo; Michael Kasimatis; Yasin Cotur; Tarek Asfour; Alex Collins; Ugur Tanriverdi; Max Grell; Matti Kaisti; Guglielmo Senesi; Karen Stevenson; Firat Guder
Title: Ultra-Low-Cost Integrated Silicon-based Transducer for On-Site, Genetic Detection of Pathogens Document date: 2020_3_25
ID: 7a3wdduq_4
Snippet: There are several molecular methods to amplify NA for detection with high specificity e.g., polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), stranddisplacement amplification (SDA), recombinase-polymerase amplification (RPA). While PCR requires thermocycling; emerging isothermal methods such as LAMP and RPA, do not, removing the need for sophisticated instruments. 2, 3 On the other hand, PCR is a simple process, requ.....
Document: There are several molecular methods to amplify NA for detection with high specificity e.g., polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), stranddisplacement amplification (SDA), recombinase-polymerase amplification (RPA). While PCR requires thermocycling; emerging isothermal methods such as LAMP and RPA, do not, removing the need for sophisticated instruments. 2, 3 On the other hand, PCR is a simple process, requiring few reagents. It is also the gold standard for NA-based laboratory diagnostics with a solid support infrastructure. Amplification strategies are traditionally combined with fluorescence-based optical methods of detection to produce a quantitative analytical signal. Optical methods, even though author/funder. All rights reserved. No reuse allowed without permission.
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