Under-the-radar dengue virus infections in natural populations of Aedes aegypti mosquitoes

Sean M. Boyles; Carla N. Mavian; Esteban Finol; Maria Ukhanova; Caroline J. Stephenson; Gabriela Hamerlinck; Seokyoung Kang; Caleb Baumgartner; Mary Geesey; Israel Stinton; Kate Williams; Derrick K. Mathias; Mattia Prosperi; Volker Mai; Marco Salemi; Eva A. Buckner; John A. Lednicky; Adam R. Rivers; Rhoel R. Dinglasan

To estimate the most recent common ancestor (MRCA) for DENV4 entry into Manatee County, 182 Florida, as well as date divergence of the strain with Haitian isolates, we performed a 183 molecular clock analysis using a Bayesian evolutionary framework [29] on a reduced dataset 184 including only the "Americas clade." We first assessed the phylogenetic signal and the 185 absence of nucleotide substitution saturation ( Fig. S2c-d) and then the temporal signal alone 186 (Fig. S3) . In the maximum clade credibility (MCC) tree, Manatee DENV4 clustered with the 187 Haitian isolates from 2014 (Node A posterior probability [PP] > 0.9) (Fig. 3b) . The MCC 188 author/funder. All rights reserved. No reuse allowed without permission. Haitian isolates was 2010 (Node A in Fig. 3b ). This 95% high posterior density interval for this 210 We then explored selective pressures on the Manatee County DENV4 strain's coding 211 sequence that may be functionally important with respect to transmission and persistence of 212 author/funder. All rights reserved. No reuse allowed without permission.