Author: Daniela Weiskopf; Katharina S. Schmitz; Matthijs P. Raadsen; Alba Grifoni; Nisreen M.A. Okba; Henrik Endeman; Johannes P.C. van den Akker; Richard Molenkamp; Marion P.G. Koopmans; Eric C.M. van Gorp; Bart L. Haagmans; Rik L. de Swart; Alessandro Sette; Rory Dylan de Vries
Title: Phenotype of SARS-CoV-2-specific T-cells in COVID-19 patients with acute respiratory distress syndrome Document date: 2020_4_18
ID: 97gme3pl_2
Snippet: Despite the large numbers of cases and deaths, no information is available on the phenotype of SARS-CoV-2-specific T-cells. Here, we stimulated peripheral blood mononuclear cells (PBMC) from eight COVID-19 patients with ARDS, collected up to three weeks after admission to the intensive care unit (ICU), with MegaPools (MP) of overlapping or prediction-based peptides covering the SARS-CoV-2 proteome 7 . We detected SARS-CoV-2-specific CD4 + and CD8.....
Document: Despite the large numbers of cases and deaths, no information is available on the phenotype of SARS-CoV-2-specific T-cells. Here, we stimulated peripheral blood mononuclear cells (PBMC) from eight COVID-19 patients with ARDS, collected up to three weeks after admission to the intensive care unit (ICU), with MegaPools (MP) of overlapping or prediction-based peptides covering the SARS-CoV-2 proteome 7 . We detected SARS-CoV-2-specific CD4 + and CD8 + T-cells in all COVID-19 patients, whereas peptide stimulation of healthy control PBMC samples collected before the outbreak showed no response. SARS-CoV-2-specific T-cells predominantly produced Th1 cytokines, although Th2 cytokines were also detected.
Search related documents:
Co phrase search for related documents- ARDS patient and intensive care: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45
- ARDS patient and intensive care unit: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32
- ARDS patient and large number: 1
- ARDS patient and SARS detect: 1
- blood mononuclear and control PBMC: 1, 2, 3, 4, 5
- blood mononuclear and ICU intensive care unit: 1, 2, 3, 4, 5, 6
- blood mononuclear and intensive care: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17
- blood mononuclear and intensive care unit: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
- blood mononuclear and large number: 1, 2, 3, 4
- blood mononuclear and patient cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
- blood mononuclear and PBMC blood mononuclear cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54
- blood mononuclear and peptide stimulation: 1, 2, 3, 4, 5, 6, 7
- blood mononuclear and peripheral PBMC blood mononuclear cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55
- blood mononuclear and response show: 1, 2, 3, 4, 5, 6, 7, 8
- blood mononuclear and SARS detect: 1
- blood mononuclear and th1 cytokine: 1, 2, 3, 4, 5, 6, 7
- blood mononuclear cell and intensive care: 1, 2
- blood mononuclear cell and intensive care unit: 1, 2
- blood mononuclear cell and large number: 1
Co phrase search for related documents, hyperlinks ordered by date